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Subcellular distribution of prostaglandin-E2 and prostaglandin-F2alpha in atrial tissue from patients with mitral valve disease
Zamorano, B; Escobar, E; Gazmuri, R.
  • Zamorano, B; University of Chile. Faculty of Medicine. Salvador Hospital. Department of Physiology and Biophysics.
  • Escobar, E; University of Chile. Faculty of Medicine. Salvador Hospital. Section of Cardiology.
  • Gazmuri, R; University of Chile. Faculty of Medicine. Salvador Hospital. Section of Cardiology.
Biol. Res ; 31(4): 343-9, 1998. tab, ilus, graf
Artigo em Inglês | LILACS | ID: lil-226035
ABSTRACT
The distribution of prostaglandin-E2 (PGE2) and prostaglandin-F2alpha (PGF2alpha) was studied in subcellular fractions isolated from homogenates of human atrial fresh tissue by differential centrifugation. Right and left atrial samples were excised from the same heart of six patients with mitral valve disease at the time of open heart surgery. The atrial fractions investigated were mitochondrial (8,500 g pellet), microsomal (100,000 g pellet) and cytosol soluble (100,000 g supernatant) fractions. After extraction of prostaglandins from the three atrial fractions and separation of PGE from PGF series by chromatography on silicic acid column, these prostaglandins were measured by radioimmunoassay. The results, showed that PGE2 and PGF2alpha were located mainly in the soluble cytosolic fraction of right and left atrial tissue (p<0.001). Furthermore, the prostaglandins levels were higher in left than in right atria of these patients (p<0.001). The relation between prostaglandins heart generation in response to elevated work load of mitral valve disease is discussed.
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Índice: LILACS (Américas) Assunto principal: Ocitócicos / Dinoprosta / Dinoprostona / Insuficiência da Valva Mitral / Estenose da Valva Mitral Limite: Adulto / Feminino / Humanos / Masculino Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 1998 Tipo de documento: Artigo / Documento de projeto

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Índice: LILACS (Américas) Assunto principal: Ocitócicos / Dinoprosta / Dinoprostona / Insuficiência da Valva Mitral / Estenose da Valva Mitral Limite: Adulto / Feminino / Humanos / Masculino Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 1998 Tipo de documento: Artigo / Documento de projeto