Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions
Braz. j. med. biol. res
;
32(1): 39-43, Jan. 1999. graf
Artigo
em Inglês
| LILACS
| ID: lil-226210
ABSTRACT
The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64 percent at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38 percent. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21 percent when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with changes in the molecular conformation of the zymogen. This effect is strongly dependent on the presence of a fibrin clot
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plasminogênio
/
Metaloproteases
/
Fibrinolíticos
/
Íons
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1999
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS