A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
Braz. j. med. biol. res
;
32(1): 45-9, Jan. 1999. ilus
Artigo
em Inglês
| LILACS
| ID: lil-226211
RESUMO
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4 percent) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Amplificação de Genes
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Genótipo
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1999
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS