Detection of point mutations by non-isotopic single strand conformation polymorphism
Braz. j. med. biol. res
;
32(1): 55-8, Jan. 1999. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-226213
RESUMO
We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Neoplasias Gástricas
/
Neoplasias do Colo do Útero
/
Genes p53
/
Mutação Puntual
/
Polimorfismo Conformacional de Fita Simples
/
Neoplasias de Cabeça e Pescoço
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1999
Tipo de documento:
Artigo
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