Molecular mechanisms of angiogenesis: experimental models define cellular trafficking of FGF-1
P. R. health sci. j
;
15(3): 179-86, Sept. 1996.
Artigo
em Inglês
| LILACS
| ID: lil-228509
ABSTRACT
Numerous studies have established that stimulation of cell growth by members of the fibroblast growth factor (FGF) family of polypeptides is dependent upon an extracellular pathway. Acidic FGF (FGF-1), however, lacks a classical signal sequence for secretion, thereby making it difficult to evaluate regulation of biological activity by this growth factor. Efforts in this laboratory have utilized molecular techniques of retrovirology and transgenic modeling to introduce cDNA sequences encoding either an intracellular or extracellular form of FGF-1 into primary diploid cells to examine trafficking and compartmentalization of FGF-1. Several lines of evidence obtained from these models provide a compelling argument that the stimulation of FGF-1-associated cellular transformation is restricted to an extracellular, receptor-mediated pathway, involving protein tyrosine phosphorylation and nuclear localization. In addition, an unconventional secretion pathway for intracellular FGF-1 has been identified that involves mechanisms associated with oxidative stress
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Índice:
LILACS (Américas)
Assunto principal:
Transdução de Sinais
/
Fator 1 de Crescimento de Fibroblastos
/
Neovascularização Fisiológica
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
P. R. health sci. j
Assunto da revista:
Medicina
Ano de publicação:
1996
Tipo de documento:
Artigo
País de afiliação:
Brasil
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