Expression of interferons-alpha and -beta, tumor necrosis factor-alpha and transcription factors, IRF-1 and IRF-2, in leukocytes induced during interferon production
Biol. Res
;
28(4): 283-90, 1995.
Artigo
em Inglês
| LILACS
| ID: lil-228573
ABSTRACT
Analysis of gene expression in peripheral blood lymphocytes is of special interest because it could reflect physiological conditions. We have examined the expression and compared the relative amounts of specific mRNAs for interferons (IFN-alpha and IFN-beta), tumor necrosis factor-alpha (TNF-alpha) and interferon regulatory factors (IRF-1 and IRF-2) from interferon primed and Sendai virus induced peripheral blood leukocytes. Results obtained showed that IRF-1 was highly inducible by IFN treatment, IFN-alpha, TNF-alpha and IRF-2 were weakly induced by IFN treatment, and IFN-beta was not inducible by priming the cells with recombinant human IFN-alpha 2b. The IFN-alpha, IFN-beta, IRF-2 and TNF-alpha transcripts increased upon viral infection. The IRF-1 mRNA was rapidly induced by IFN treatment and decreased after Sendai virus infection. Our results show that, in peripheral blood lymphocytes, IFN-alpha and -beta genes have a different response to IFN induction, thus suggesting different regulatory mechanisms for IFN induction of type I IFN genes in peripheral blood lymphocytes
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Índice:
LILACS (Américas)
Assunto principal:
Fosfoproteínas
/
Fatores de Transcrição
/
Expressão Gênica
/
Fator de Necrose Tumoral alfa
/
Interferon beta
/
Interferon-alfa
/
Proteínas de Ligação a DNA
/
Leucócitos
Limite:
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
1995
Tipo de documento:
Artigo
País de afiliação:
Brasil
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