Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography
Biol. Res
;
26(1/2): 101-7, 1993. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-228595
RESUMO
The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity
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Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Cisteína Proteases
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
1993
Tipo de documento:
Artigo
País de afiliação:
Argentina
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