The separation and identification of picomole amounts of intermediates of glucose metabolism by high performance liquid chromatography on pellicular resins
Biol. Res
;
25(2): 73-8, 1992. graf
Artigo
em Inglês
| LILACS
| ID: lil-228636
ABSTRACT
A column (CarboPac PA1, Dionex) containing an anion-exchange pellicular resin was used for the separation of phosphoryl-hexoses derived from labeled glucose microinjected into individual frog oocytes or from cultures of Escherichia coli. Intermediates were identified by a) comparison of retention times with those of authentic commercial compounds; b) the use of internal labeled standards; c) incubation of samples with specific enzymes and noting the disappearance of one radioactive peak and appearance of another at a new retention time
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Índice:
LILACS (Américas)
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Glucose
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
1992
Tipo de documento:
Artigo
País de afiliação:
Brasil
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