An improved histochemical technique for differentiating adrenaline-and noradrenaline-containing cells
Arch. med. res
;
29(1): 75-7, ene.-mar. 1998. ilus
Artigo
em Inglês
| LILACS
| ID: lil-232619
ABSTRACT
Background. Sevki's histochemical technique allows specific staining of catechomamine-containing cells, yet discrimiantion between adrenaline-(ADR-) cells and noradrenaline-(NOR-) cells is unrealiable, being based on hue differences. Methods. In this work, histochemical differentiation of ADR- and NOR-cells in rat adrenal medulla was carried out by introducing two modifications to Sevki's technique: 1) employment of aged Giemsa solution, and 2) addition of an alkaline differentiating step. Results. With these changes, ADR-cell stained brown, whereas NOR-cells were deep-green, resulting in a clear-cut differentiation. Conclusions. The modified technique permits to differentiate ADR- from NOR-cells in the adrenal medulla using only a bright field microscope without any sophisticated equipment. The present procedure is inexpensive and easy to carry out
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Índice:
LILACS (Américas)
Assunto principal:
Epinefrina
/
Norepinefrina
/
Diferenciação Celular
/
Ratos Wistar
/
Medula Suprarrenal
/
Histocitoquímica
Limite:
Animais
Idioma:
Inglês
Revista:
Arch. med. res
Assunto da revista:
Medicina
Ano de publicação:
1998
Tipo de documento:
Artigo
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