A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus
Braz. j. med. biol. res
;
32(6): 747-52, Jun. 1999. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-233707
RESUMO
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88 per cent), specificity (100 per cent) and agreement (95.31 per cent).
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vírus da Bronquite Infecciosa
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1999
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
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