Hunting for differentially expressed genes
Braz. j. med. biol. res
;
32(7): 877-84, July 1999.
Artigo
em Inglês
| LILACS
| ID: lil-234894
ABSTRACT
Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Expressão Gênica
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Genes
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1999
Tipo de documento:
Artigo
/
Congresso e conferência
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