Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase / Dot-ELISA (dot enzyme-linked immunosorbent assay), utilizando o dacron como suporte sólido para o diagnóstico da esquistossomose
Rev. Soc. Bras. Med. Trop
;
32(2): 139-43, mar.-abr. 1999. tab
Artigo
em Inglês, Português
| LILACS
| ID: lil-235183
RESUMO
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ between nitrocellulose and dacron. Specificity was lower when dacron was used than when nitrocellulose was used, although the difference was not statistically significant (p > 0.05). In conclusion, this work showed that nitrocellulose and dacron performed similarly in dot-ELISA, suggesting that they may be used altenatively in population surveillance in endemic areas
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Schistosoma mansoni
/
Esquistossomose mansoni
/
Anticorpos Anti-Helmínticos
/
Colódio
/
Filtros Microporos
/
Antígenos de Helmintos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
/
Português
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Brasil
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