Cell envelope components of Yersinia pestis grown in intraperitoneal diffusion chambers
Rev. microbiol
;
29(3): 174-8, jul.-set. 1998. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-236203
ABSTRACT
The electrophoretic profiles binding of penicillin binding proteins (PBPs) and outer membrane proteins (OMPs) of Yersinia pestis EV 76 were determined following in vivo growth in diffusion chambers implanted in the peritoneal cavity of mice. In contrast to Y. pestis grown under in vitro conditions which activate the low calcium response (LCR) regulon there was no significant qualitative or quantitative change of the PBP profile of Y. pestis cells during growth in diffusion chambers for up to 72 h following implatation in mice. Three OMPs, with molecular weight of 100, 60 and 58 kDa, were expressed in Y. pestis cells grown for 24 h, but not at 48 h or at 72 h, in diffusion chambers. These results indicate that growth of Y. pestis in intraperitoneal diffusion chambers activates genes which might be relevant to the growth in the mammal host.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Penicilinas
/
Yersinia pestis
/
Proteínas de Transporte
/
Proteínas de Membrana
Tipo de estudo:
Pesquisa qualitativa
Limite:
Animais
Idioma:
Inglês
Revista:
Rev. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
1998
Tipo de documento:
Artigo
País de afiliação:
Brasil
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