Calibration and use of an in-house anti-measles IgG standard serum
Rev. Inst. Med. Trop. Säo Paulo
;
41(1): 13-20, Jan.-Feb. 1999. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-236720
RESUMO
For the purpose of research a large quantity of anti-measles IgG working reference serum was needed. A pool of sera from five teenagers was prepared and named Alexandre Herculano (AH). In order to calibrate the AH serum, 18 EIA assays were performed testing in parallel AH and the 2nd International Standard 1990, Anti-Measles Antibody, 66/202 (IS) in a range of dilutions (from 1/50 to 1/25600). A method which compared parallel lines resulting from the graphic representation of the results of laboratory tests was used to estimate the power of AH relative to IS. A computer programme written by one of the authors was used to analyze the data and make potency estimates. Another method of analysis was used, comparing logistic curves relating serum concentrations with optical density by EIA. For that purpose an existing computer programme (WRANL) was used. The potency of AH relative to IS, by either method, was estimated to be 2.4. As IS has 5000 milli international units (mIU) of anti-measles IgG per millilitre (ml), we concluded that AH has 12000 mIU/ml.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Padrões de Referência
/
Calibragem
/
Vacina contra Sarampo
Tipo de estudo:
Estudo prognóstico
Limite:
Adolescente
/
Humanos
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
/
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