Anti-Inflammatory activity od debaryomyces hansenii Cu, Zn-SOD

RESUMO

Background. Cu,Zn-superoxide-dismutase, Cu,Zn-SOD, can be obtained from different sources with different anti-inflammatory activities. In this study we compared the antiinflammatory capacity of the marine yeast Debaryomyces hanseii Cu,Zn-SOD (Dh-SOD) with that of bovine erythrocytes (Be-SOD) in preventive an a therapeutic fashion. Methods. Edema was induced by carrageenan injection into the rat hind paw and was evaluated using a mercury plethysmograph. Development of the inflammatory process was followed by volume displacement at time 0 (carrageenan injection), 1, 2, 3, 4, 5, 6, 9, 12, and 24 h thereafter. Three different SOD doses were used in preliminary experiments to prevent edema 10, 100, and U/kg. Results. The results indicate that, at the lowest dose (10 U/kg), both SOD samples are effective in reducing inflammation in both the prostaglandin and amplification phases (-24.8 percent and -17.5 percent in the case of Be-SOD, and 11.8 percent and -18.7 percent in the case of Dh-SOD, respectively) (p<0.05). At 100 U/kg, Be-SOD also shows good anti-inflammatory activity in all edema phases (-27.1 percent in the serotonin phase; -19.4 percent in the prostaglandin phase; and -20 percent in the amplification phase) (p<0.05), but Dh-SOD was less effective (-10.9 percent, -9.1 percent, and -5.7 percent). At the highest dose tested (1000 U/kg), Dh-SOD was, again more effective than Be-SOD in all three edema phases (-33.1 percent and -1.5 percent; -17.9 percent and -2.6 percent; and -13.8 percent and 6.7 percent, respectively) (p >0.05). When evaluated as a therapeutic alternative, single doses of DH-SOD at 1,000 U/kg, and Be-SOD at 100 U/kg, both showed good anti-inflammatory activities (-31.7 percent and -23.5 percent, respectively) (p < 0.05). Conclusion. For therapy purposes alone, DH-SOD appears to be a better anti-inflammatory agent than Be-SOD in carrageenan-induced edema