Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
Braz. j. med. biol. res
;
33(11): 1269-74, Nov. 2000. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-273211
ABSTRACT
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eight Enterococcus faecium isolates from Stanford University and 12 from Sao Paulo Hospital were studied by JB1-PCR, REP-PCR 1/2R and PFGE. Among the isolates from Stanford University, 5 genotypes were defined by JB1-PCR, 7 by REP-PCR 1/2R and 4 by PFGE. Among the isolates from Sao Paulo Hospital, 9 genotypes were identified by JB1-PCR, 6 by REP-PCR and 5 by PFGE. The three methods identified identical genotypes, but there was not complete agreement among them
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
DNA Bacteriano
/
Enterococcus faecium
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Maringá/BR
/
Universidade Federal de Säo Paulo/BR
Similares
MEDLINE
...
LILACS
LIS