Mutación del gen p53 en el cáncer de colon y recto / P53 gene mutation in colorectal cancer
Rev. méd. Chile
;
128(9): 996-104, sept. 2000. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-274632
ABSTRACT
Background:
Genetic events associated to colorectal carcinoma are well characterized, but there is scanty information about this issue in Chilean subjects.Aim:
To determine the frequency and distribution of exons 5, 6, 7, 8 and 9 mutations and the immunohistochemical expression of p53 gene in biopsy samples of colorectal carcinoma. Material andmethods:
p53 gene exons 5, 6, 7, 8 and 9 were directly sequenced in 42 biopsy samples of colorectal carcinoma. Immunohistochemical expression of p53 was determined in 35 samples.Results:
Thirty one discrete mutations (12 transitions, 11 transversions and 8 insertions) were observed in 21 samples (60 percent). Nine samples had mutations in exon 5, twelve samples had mutations in exon 6, seven samples had mutations in exon 7 and three samples had mutations in exons 8 and 9. Immunohistochemical expression of p53 protein was observed in 18 of 35 cases. There was a high correlation between the genetic alteration and immunohistochemistry, when p53 was expressed in more the 20 percent of cells. The positive and negative predictive values of p53 expression were 87 and 80 percent respectively. There was a non significant lower mortality among patients with mutations in their biopsies.Conclusions:
These results confirm the involvement of p53 gene mutations in colonic carcinogenesis. Immunohistochemical methods for the detection of p53 protein have a high predictive value
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Neoplasias Colorretais
/
Adenocarcinoma
/
Genes p53
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2000
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
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