A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses
Mem. Inst. Oswaldo Cruz
;
96(6): 865-873, Aug. 2001. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-298603
ABSTRACT
A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20 percent fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10 percent fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Arbovírus
/
Linhagem Celular
/
Culicidae
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2001
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Instituto Nacional de Salud/CO
/
Universidad De La Salle/CO
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