Influence of spore concentration on microorganism morphology and on glucoamylase production in submerged cultures of Aspergillus awamori
Braz. j. microbiol
;
31(supl.1): 49-54, Oct. 2000. tab
Artigo
em Inglês
| LILACS
| ID: lil-300567
ABSTRACT
The influence of the spore concentration in the inoculum preparation on microorganism morphology and glucoamylase synthesis by submerged cultures of Aspergillus awamori was investigated. A series of fermenter runs were perfomed, using an initial total reducing sugar concentration of 40 g/L. The inocula were prepared in a rotatory shaker, at 35§C 200 rev/min for 24 hours, varying spore concentration in the flasks from 9.5 x 10(3) to 1.8 x 10 (7) spores/mL. The inocula prepared with a spore concentration between 9.5 x 10(3) spores/mL and 5.0 x 10(6) spores/mL were composed by a cell suspension mainly in the pellet form, which led to a filamentous growth in the fermenter. Glucoamylase production was not affected by this range of spore concentration, reaching values between 2,100 U/L and 2,300 U/L. However, a higher spore concentration in the inoculum preparation (1.8 x 10(7) spores/mL) led to a inoculum formed by a cell suspension in the filamentous form containing many spore agglomerates (non-germinated spores). The kind of inoculum led to a growth predominantly in the pellet form in fermenter, which reduced the glucoamylase production in 30 (per cent).
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Índice:
LILACS (Américas)
Assunto principal:
Aspergillus
/
Técnicas In Vitro
/
Ensaios Enzimáticos Clínicos
/
Enzimas
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Escola Politécnica da USP. Depto. de Engenharia Química/BR
/
Universidade Estadual de Maringá. Depto. de Engenharia Química/BR
/
Universidade Federal de São Carlos. Departamento de Engenharia Quimica/BR
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