Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant
Biol. Res
;
34(3/4): 227-236, 2001. ilus, tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-303886
RESUMO
Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration beta-MAAC). Methylcellulose discs instilled with 10 microliters of beta-MAAC (0.08 microgram/ml), 10 microliters of tumor supernatant (TA3ts), 5 microliters beta-MAAC + 5 microliters TA3ts, and 10 microliters of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 micron 2). The results show that beta-MAAC produced a significant inhibition of neovascularization in comparison to that observed in controls (P < 0.0025; Student t-Test). Discs instilled with TA3ts produced an intense stimulation of angiogenesis in contrast, when discs were instilled with 5 microliters of beta-MAAC + 5 microliters of TA3ts the angiogenesis was significantly inhibited (P < 0.001). The results show that effective antiangiogenic doses of betamethasone are in the range of 10(-7) M, (probably a genomic mediated action) and that this effect of low concentration may have clinical applications.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Betametasona
/
Inibidores da Angiogênese
/
Alantoide
/
Neovascularização Patológica
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2001
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Instituto de Salud Pública de Chile/CL
/
Universidad de Chile/CL
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