A heminested polymerase chain reaction for the detection of Brazilian rabies isolates from vampire bats and herbivores
Mem. Inst. Oswaldo Cruz
;
97(1): 109-111, Jan. 2002. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-306075
ABSTRACT
A heminested-PCR (hn-PCR) using primers to the nucleoprotein-coding gene in a nested set was evaluated in the detection of Brazilian strains of rabies virus (RV). A representative number of RV nucleoprotein sequences belonging to genotype 1 were aligned. Based on such alignment, primers were directed to highly conserved regions. All 42 clinical samples positive by both fluorescent antibody and mouse inoculation tests were also positive by the hn-PCR. Brain tissue that had been left to decompose, obtained from an experimentally inoculated mouse was tested by hn-PCR and yielded positive results. In conclusion, primers designed here were capable of amplifying Brazilian RV isolates obtained from a rural epidemiological cycle
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Raiva
/
Vírus da Raiva
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Santo Amaro/BR
/
Universidade de Säo Paulo/BR
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