Metodología clásica y molecular en la identificación de especies de enterococcus spp / Classic and molecular methodologies for the identification of enterococcus species
Rev. méd. Chile
;
130(1): 45-49, ene. 2002. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-310251
RESUMO
Background:
Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial.Aim:
To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). Material andmethods:
Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR.Results:
Eighty nine percent of isolates were identified as E fócalis, 10.2 percent as E fócium and 3.3 percent as other species.Conclusions:
PCR is a fast and promising technique, useful in the identification of Enterococcus species
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Reação em Cadeia da Polimerase
/
Enterococcus
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
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