Partial purification and characterization of exopolygalacturonase II and III of penicillium frequentans
Braz. j. microbiol
; 32(4): 327-330, Oct.-Dec. 2001. tab, graf
Article
em En
| LILACS
| ID: lil-314807
Biblioteca responsável:
BR32.1
RESUMO
Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDA (Exo-PG II) and 79 kDA (Exo- PG III). The K(m) values were 1.6 and 0.059 g/L and the V(max) values were 2571 and 185 U/mg, respectively. The optimim temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
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LILACS
Assunto principal:
Penicillium
/
Poligalacturonase
/
Técnicas In Vitro
/
Ensaios Enzimáticos Clínicos
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Fungos
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2001
Tipo de documento:
Article