Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región Chile / Detection of BCR-ABL gene sequences using polymerase chain reaction reverse transcriptase in patients with leukemia from the IX Región of Chile
Rev. méd. Chile
;
130(6): 623-630, jun. 2002. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-317493
ABSTRACT
Background:
The BCR-ABL fusion gene is the molecular expression of the Philadelphia chromosome. This cytogenetic aberration is the most frequent alteration found in leukemias, which is produced by the translocation t(9;22). Two different fusion proteins are produced depending on the break point (210 kD and 190 kD). The detection of this gene has both diagnostic and prognostic importance, associated with poor prognosis in acute lymphoblastic leukemia (ALL).Aim:
To detect BCR-ABL gene sequences in patients with leukemia from the IX Region of Chile. Material andmethods:
We studied 58 patients 5 chronic myeloid leukemia (CML), 35 ALL, 15 acute myeloid leukemia (AML) and 3 biphenotypic leukemia. The gene sequences were detected using reverse transcriptase polymerase chain reaction (RT-PCR).Results:
BRC-ABL gene sequences were positive in all patients with CML, 2 of 35 ALL (one child and one adult). The remaining patients were negative. We found p210 and p190 co-expression in 2 CML and 1 ALL.Conclusions:
Our results are in agreement with other reports. The detection of these and other genetic alterations will allow us to have invaluable diagnostic and prognostic information from our patients with leukemia
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Leucemia Mieloide
/
Proteínas de Fusão bcr-abl
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Adolescente
/
Adulto
/
Criança
/
Criança, pré-escolar
/
Humanos
/
Lactente
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
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