An oligonucleotide probe derived from kDNA minirepeats is specific for Leishmania (Viannia)
Mem. Inst. Oswaldo Cruz
;
91(3): 279-284, May-Jun. 1996.
Artigo
em Inglês
| LILACS
| ID: lil-319872
RESUMO
Sequence analysis of Leishmania (Viannia) kDNA minicircles and analysis of multiple sequence alignments of the conserved region (minirepeats) of five distinct minicircles from L. (V.) braziliensis species with corresponding sequences derived from other dermotropic leishmanias indicated the presence of a sub-genus specific sequence. An oligonucleotide bearing this sequence was designed and used as a molecular probe, being able to recognize solely the sub-genus Viannia species in hybridization experiments. A dendrogram reflecting the homologies among the minirepeat sequences was constructed. Sequence clustering was obtained corresponding to the traditional classification based on similarity of biochemical, biological and parasitological characteristics of these Leishmania species, distinguishing the Old World dermotropic leishmanias, the New World dermotropic leishmanias of the sub-genus Leishmania and of the sub-genus Viannia.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Oligonucleotídeos
/
DNA de Cinetoplasto
/
Leishmania
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
1996
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Panamá
Instituição/País de afiliação:
Instituto Oswado Cruz/BR
/
Instituto Oswaldo Cruz/BR
/
Universidad de Panamá/PA
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade do Estado do Rio de Janeiro/BR
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