Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of internal transcriber spacer of the RNA ribosomal gene
Mem. Inst. Oswaldo Cruz
;
95(2): 171-177, Mar.-Apr. 2000.
Artigo
em Inglês
| LILACS
| ID: lil-319979
RESUMO
Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Morphological identification was carried out by comparison of characters of the shell and the male and female reproductive apparatus. The PCR-RFLP involved amplification of the internal spacer region ITS1 and ITS2 of the RNA ribosomal gene and subsequent digestion of this fragment by the restriction enzymes DdeI, MnlI, HaeIII and MspI. The planorbids were compared with snails of the same species and others reported from Venezuela and present in Brazil, Cuba and Mexico. All the enzymes showed a specific profile for each species, that of DdeI being the clearest. The snails were identified as B. glabrata, B. prona and B. kuhniana.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Biomphalaria
/
Polimorfismo de Fragmento de Restrição
/
RNA Ribossômico
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Venezuela
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Centro de Pesquisas René Rachou. Fundação do Instituto Oswaldo Cruz/BR
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