Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies
Rev. Inst. Med. Trop. Säo Paulo
;
35(2): 171-175, Mar.-Apr. 1993.
Artigo
em Inglês
| LILACS, SES-SP
| ID: lil-320572
RESUMO
A simplified fluorescence inhibition microtest (SFIMT) was standardized for the evaluation of antirabies serum neutralizing antibodies based on the rapid fluorescent focus inhibition test (RFFIT) and the fluorescence inhibition microtest (FIMT). The simplified test showed reproducibility similar to that of the FIMT with advantages as easier executation and quicker reading. A simple pre-treatment of Brazilian microplates produced for immune enzymatic assays (PROSIL) gave equivalent results and substantial coast reduction, in relation to imported plates (DIFCO). The simplified test can be easily implemented in less sophisticated laboratories, as alternative to the mouse serum neutralization test, still the most largely employed in Brazil, or even to others as RFFIT and FIMT.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vírus da Raiva
/
Imunofluorescência
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Ano de publicação:
1993
Tipo de documento:
Artigo
Instituição/País de afiliação:
Escola Paulista de Medicina/BR
/
Instituto Pasteur/BR
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