UltramicroELISA indirecto para la detección de anticuerpos totales a Citomegalovirus en suero humano / Indirect ultramicroElisa for the detection of total antibodies against cytomegaloviruses in human blood
Rev. Inst. Med. Trop. Säo Paulo
;
34(1): 43-47, Jan.-Feb. 1992.
Artigo
em Espanhol
| LILACS
| ID: lil-320631
RESUMO
We have standardized an indirect ultramicro ELISA assay for detecting antibodies to human Cytomegalovirus (CMV) using human serum samples (UMELISA CMV). The optimal concentration of coating antigen (30 ug/ml), serum dilution (140) and anti-human conjugate working dilution (11500), were determined by a check board titration method. The UMELISA CMV was compared with the latex agglutination test for antibodies to CMV (Dupont de Nemours) and with an indirect immunofluorescent method. The results have showed the high coincidence, sensitivity and especificity of the proposed assay regarding the two methods compared with, and supporting its use either for a blood donors screening or in the serological diagnosis of this infection by paired serum samples.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Citomegalovirus
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Espanhol
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
1992
Tipo de documento:
Artigo
País de afiliação:
Cuba
Instituição/País de afiliação:
Banco de Sangue Provincial/CU
/
Centro de Inmunoensayo/CU
/
Instituto de Medicina Tropical Pedro Kouri Siboney/CU
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