Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (viannia) braziliensis
Rev. Inst. Med. Trop. Säo Paulo
;
38(3): 177-185, May-Jun. 1996.
Artigo
em Inglês
| LILACS, SES-SP
| ID: lil-320648
ABSTRACT
We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100 positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100 positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100 positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95 of Kala-azar and 35 of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1512. In contrast, 15 of Chagas sera agglutinated the parasite in dilutions 116 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Anticorpos Antiprotozoários
/
Leishmaniose Mucocutânea
/
Doença de Chagas
/
Leishmaniose Visceral
Limite:
Animais
/
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Ano de publicação:
1996
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidade de Brasilia/BR
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