Modelo experimental de transfecção gênica em miocárdio normal de cães: perspectivas de terapia gênica para o tratamento da cardiopatia isquêmica / Experimental model of gene transfection in healthy canine myocardium: perspectives of gene therapy for ischemic heart disease
Arq. bras. cardiol
;
79(3): 223-232, Sept. 2002. ilus
Artigo
em Português, Inglês
| LILACS
| ID: lil-321665
ABSTRACT
OBJECTIVE:
To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection.METHODS:
The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline solution (n=1) or 0.5 mL of plasmid solution containing 0.5 æg of pREGFP/dog (n=4) were injected into the myocardium of the left ventricular lateral wall. The dogs were euthanized 1 week later, and cardiac biopsies were obtained.RESULTS:
Fluorescence microscopy showed differences between the cells transfected and not transfected with pREGFP plasmid. Mild fluorescence was observed in the cardiac fibers that received saline solution; however, the myocardial cells transfected with pREGFP had overt EGFP expression.CONCLUSION:
Transfection with the EGFP gene in healthy canine myocardium was effective. The reproduction of this efficacy using vascular endothelial growth factor (VEGF) instead of EGFP aims at developing gene therapy for ischemic heart disease
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Transfecção
/
Terapia Genética
/
Isquemia Miocárdica
/
Proteínas Luminescentes
/
Modelos Genéticos
/
Miocárdio
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
/
Português
Revista:
Arq. bras. cardiol
Assunto da revista:
Cardiologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundaçäo Universitária de Cardiologia/BR
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