A simple and inexpensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp
Rev. Inst. Med. Trop. Säo Paulo
;
38(3): 241-242, May-Jun. 1996.
Artigo
em Inglês
| LILACS
| ID: lil-321804
ABSTRACT
Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x 8 in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2) of samples incubated in jars with gas generator envelopes and from 129 (98) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Campylobacter
/
Técnicas Microbiológicas
Limite:
Animais
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
1996
Tipo de documento:
Artigo
País de afiliação:
Costa Rica
Instituição/País de afiliação:
University of Costa Rica/CR
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