Genetic instability of sugarcane plants derived from meristem cultures
Genet. mol. biol
;
25(1): 91-96, 2002. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-324993
ABSTRACT
The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93 percent when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Plantas
/
Variação Genética
/
Técnicas de Amplificação de Ácido Nucleico
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Centro de Tecnologia Copersucar/BR
/
Escola Superior de Agricultura "Luiz de Queiroz"/BR
/
Universidade Estadual de Londrina/BR
/
Universidade Federal de Säo Carlos/BR
Similares
MEDLINE
...
LILACS
LIS