Differentiation of mouse embryonic stem cells and their hybrids during embryoid body formation
Genet. mol. biol
;
25(1): 103-111, 2002. ilus, tab
Artigo
em Inglês
| LILACS, SES-SP
| ID: lil-324995
ABSTRACT
We studied the karyotypes of three hybrid clones of mouse embryonic stem cells and murine splenocytes (two having near diploid and one having near tetraploid chromosome numbers) and the characteristics of their differentiation during the formation of embryoid bodies. The X chromosome originating from embryonic stem cells may be lost in hybrids with a near diploid chromosome number and reprogramming of the "somatic" X may occur. The morphological data we obtained using light and electron microscopy revealed a correlation between the karyotype constitution of hybrid cells and their differentiation during the formation of embryoid bodies. At the beginning of development, the embryoid bodies derived from hybrid cells already showed an advanced degree of differentiation. The production of significant quantities of cartilage was typical for hybrid cells with near tetraploid chromosome numbers. The hybrid cells showed restricted pluripotent capacity and were already committed when they started to differentiate into embryoid bodies
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Diferenciação Celular
/
Clonagem de Organismos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Genet. mol. biol
Ano de publicação:
2002
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidade Estadual Norte Fluminense/BR
/
Universidade de Säo Paulo/BR
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