A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni
Mem. Inst. Oswaldo Cruz
;
97(suppl.1): 95-97, Oct. 2002. ilus
Artigo
em Inglês
| LILACS
| ID: lil-325030
ABSTRACT
Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From the previous sequencing of the ITS2 region, one primer was designed to anchor in the 5.8S conserved region and three other species-specific primers in the 28S region, flanking the ITS2 region. These four primers were simultaneously used in the same reaction (Multiplex-PCR), under high stringency conditions. Amplification of the ITS2 region of Biomphalaria snails produced distinct profiles (between 280 and 350 bp) for B. glabrata, B. tenagophila and B. straminea. The present study demonstrates that Multiplex-PCR of ITS2-DNAr showed to be a promising auxiliary tool for the morphological identification of Biomphalaria snails, the intermediate hosts of S. mansoni
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Schistosoma mansoni
/
Biomphalaria
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2002
Tipo de documento:
Artigo
/
Congresso e conferência
País de afiliação:
Brasil
/
Estados Unidos
Instituição/País de afiliação:
Fiocruz/BR
/
Instituto Ludwig de Pesquisa sobre o Câncer/BR
/
Universidade Federal de Minas Gerais/BR
/
University of Pensylvania/US
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