Applications of image analysis in the characterization of Streptomyces olindensis in submerged culture
Braz. j. microbiol
;
33(1): 17-21, jan.-mar. 2002. ilus, tab
Artigo
em Inglês, Português
| LILACS
| ID: lil-325361
ABSTRACT
The morphology of Streptomyces olindensis (producer of retamycin, an antitumor antibiotic) grown in submerged culture was assessed by image analysis. The morphology was differentiated into four classes pellets, clumps (or entangled filaments), branched and unbranched free filaments. Four morphological parameters were initially considered (area, convex area, perimeter, and convex perimeter) but only two parameters (perimeter and convex perimeter) were chosen to automatically classify the cells into the four morphological classes, using histogram analysis. Each morphological class was evaluated during growth c arried out in liquid media in fermenter or shaker. It was found that pellets and clumps dominated in early growth stages in fermenter (due to the inoculum coming from a shaker cultivation) and that during cultivation, the breakage of pellets and clumps caused an increase in the percentage of free filaments. The criteria of morphological classification by image analysis proposed were useful to quantify the percentage of each morphological class during fermentations and may help to establish correlations between antibiotic production and microorganism morphology.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Streptomyces
/
Técnicas In Vitro
/
Diagnóstico por Imagem
/
Morfogênese
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
/
Português
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
/
Universidade de São Paulo, São Paulo/BR
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