Myocardial antioxidant and oxidative stress changes due to sex hormones
Braz. j. med. biol. res
;
35(9): 1075-1081, Sept. 2002. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-325903
RESUMO
The purpose of the present study was to examine myocardial antioxidant and oxidative stress changes in male and female rats in the presence of physiological sex hormone concentrations and after castration. Twenty-four 9-week-old Wistar rats were divided into four groups of 6 animals each 1) sham-operated females, 2) castrated females, 3) sham-operated males, and 4) castrated males. When testosterone and estrogen levels were measured by radioimmunoassay, significant differences were observed between the castrated and control groups (both males and females), demonstrating the success of castration. Progesterone and catalase levels did not change in any group. Control male rats had higher levels of glutathione peroxidase (50 percent) and lower levels of superoxide dismutase (SOD, 14 percent) than females. Control females presented increased levels of SOD as compared to the other groups. After castration, SOD activity decreased by 29 percent in the female group and by 14 percent in the male group as compared to their respective controls. Lipid peroxidation (LPO) was assessed to evaluate oxidative damage to cardiac membranes by two different methods, i.e., TBARS and chemiluminescence. LPO was higher in male controls compared to female controls when evaluated by both methods, TBARS (360 percent) and chemiluminescence (46 percent). Castration induced a 200 percent increase in myocardial damage in females as determined by TBARS and a 20 percent increase as determined by chemiluminescence. In males, castration did not change LPO levels. These data suggest that estrogen may have an antioxidant role in heart muscle, while testosterone does not
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Hormônios Esteroides Gonadais
/
Estresse Oxidativo
/
Miocárdio
/
Antioxidantes
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
/
Congresso e conferência
País de afiliação:
Argentina
/
Brasil
/
Canadá
Instituição/País de afiliação:
Universidad de Buenos Aires/AR
/
Universidade Federal do Rio Grande do Sul/BR
/
University of Manitoba/CA
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