Histopathological and immunohistochemical study of the gastrointestinal tract from a dog naturally infected with Leishmania (Leishmania) chagasi: a case report
Arq. bras. med. vet. zootec
;
54(4): 340-344, jul.-ago. 2002. ilus
Artigo
em Inglês
| LILACS
| ID: lil-328407
ABSTRACT
Samples of stomach, duodenum, jejunun, ileum, cecum and colon were collected for Giemsa-smears ("imprints") from one asymptomatic mongrel dog, naturally infected with Leishmania (L) chagasi. Other fragments were obtained and fixed in formalin (10 percent and buffered) for histopathological and immunohistochemical studies. The immunohistochemistry was carried out by a streptavidin-peroxidase technique and it allowed to detect amastigote forms of Leishmania chagasi in the different paraffin gut sections. The principal lesion observed was a discrete to moderate chronic inflammatory reaction in the mucosa and submucosa in all fragments of the gastrointestinal tract (GIT). A chronic cellular exsudate was observed in all GIT tissues and it was composed by mononuclear cells (monocytes, plasmocytes and lymphocytes). A comparison between the two techniques showed that the immunohistochemistry study is the best method to detect amastigote forms of Leishmania
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Imuno-Histoquímica
/
Sistema Digestório
/
Cães
/
Leishmania
Limite:
Animais
Idioma:
Inglês
Revista:
Arq. bras. med. vet. zootec
Assunto da revista:
Medicina Veterinária
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Minas Gerais/BR
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