Purificación y caracterización de proteasas de Pseudomonas fluorescens y sus efectos sobre las proteínas de la leche / Purification and characterization of proteases from Pseudomonas fluorescens and their effects on milk proteins
Arch. latinoam. nutr
;
52(2): 160-166, jun. 2002.
Artigo
em Espanhol
| LILACS
| ID: lil-330470
RESUMO
The increase in the levels of psychotropic bacteria in the raw milk during the refrigeration period, could lead to the production of heat-resistant enzymes responsible for the deterioration of long-life industrial dairy products. Pseudomonas fluorescens is the psychotropic bacteria most commonly found in milk in Southern Chile. In the present work the enzymatic proteinases extract of cultures of Pseudomonas fluorescens RV10 at 6 degrees C in raw milk just milked were purified. It was found that the proteases corresponds to a protein with a molecular mass of 49.5 kD, that presents heat resistance and rapidly attacks the k-casein continuing with the b-casein. It is possible to conclude that storage of the milk for long-life products at 6 degrees C is risky, as it causes the loss of quality for the proteases of psychotropic bacteria.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Endopeptidases
/
Pseudomonas fluorescens
/
Leite
/
Proteínas do Leite
Limite:
Animais
Idioma:
Espanhol
Revista:
Arch. latinoam. nutr
Assunto da revista:
Bioqu¡mica
/
EducaÆo Alimentar e Nutricional
/
Fenmenos Fisiol¢gicos da NutriÆo
/
Microbiologia de Alimentos
/
NUTRICAO
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad Austral de Chile/CL
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