Enquistación in vitro de Giardia lamblia: análisis por electroforesis bidimensional de proteínas expresadas diferencialmente / In vitro encystation of Giardia lamblia: analysis with two-dimensional electrophoresis of differentially expressed proteins
Biomédica (Bogotá)
;
22(3): 253-262, sep. 2002.
Artigo
em Espanhol
| LILACS
| ID: lil-330483
RESUMO
The reconstruction of Giardia lamblia life cycle in vitro is an excellent tool for the study of the parasite's molecular biology. The present work describes techniques developed that better define parasite differentiation. An encystation protocol is presented along with a method for isolation and purification of the produced cysts. The cyst morphology at the light microscopy level is identical to that of in vivo cysts. A two-dimension protein map obtained by high-resolution electrophoresis indicated that most of the parasite's proteins are acid. Based on this result, the two dimension gel electrophoresis used a pH 4-7 gradient in the first, isoelectric focusing dimension. Differences in protein expression during the stages of encystation were clearly discerned, as well as images of the parasite obtained by light and by transmission electron microscopy that describe the morphological and the ultrastructural changes that occur as the cysts are produced in vitro.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Proteínas de Protozoários
/
Giardia lamblia
Limite:
Animais
Idioma:
Espanhol
Revista:
Biomédica (Bogotá)
Assunto da revista:
Medicina
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Instituto Nacional de Salud/CO
/
Universidad Nacional de Colombia/CO
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