Molecular typing of dengue virus type 2 in Brazil
Rev. Inst. Med. Trop. Säo Paulo
;
45(1): 17-21, Jan.- Feb. 2003. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-330507
RESUMO
Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype)
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vírus da Dengue
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
Medicina Tropical
Ano de publicação:
2003
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Estados Unidos
Instituição/País de afiliação:
Instituto Oswaldo Cruz/BR
/
University of California/US
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