Criteria for Candida albicans numerical analysis based on electrophoretic protein patterns
Rev. argent. microbiol
;
32(3): 123-128, jul.-sept. 2000.
Artigo
em Inglês
| LILACS
| ID: lil-332526
ABSTRACT
Even in normalized gels, some problems with the choice and position of protein bands always have hindered the processing of electrophoretic data. We have developed a way to establish which criterion best fits the necessities in order to maximize the similarity indexes for numerical analysis. Some repetitions of a Candida albicans strain were carried out in eleven different gels. After staining, the bands were scored in numbers within ranges of +/- n values with increases of one quarter steps (+/- 0.25 kDa, +/- 0.5 kDa, +/- 0.75 kDa, +/- 1.0 kDa, +/- 1.25 kDa) that will limit the possibility of variation for the same bands that will appear on the repetitions. Using this criterion, we have determined that values scored within +/- 1.25 kDa could optimize the minimum limiting value of similarity in different dendrograms built.
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Índice:
LILACS (Américas)
Assunto principal:
Candida albicans
/
Proteínas Fúngicas
/
Classificação
/
Densitometria
/
Eletroforese em Gel de Poliacrilamida
/
Micologia
Idioma:
Inglês
Revista:
Rev. argent. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
State University of Campinas/BR
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