Contaminación del suero fetal bovino con virus de la diarrea viral bovina / Contamination of bovine fetal serum with bovine viral diarrhea virus
Rev. argent. microbiol
;
32(1): 27-32, ene.-mar. 2000.
Artigo
em Espanhol
| LILACS
| ID: lil-332541
RESUMO
Fetal bovine serum (FBS) used in cell culture may be contaminated with viruses, among them bovine viral diarrhea virus (BVDV) affecting the production of biological reagents and the results of diagnosis. The filtration process used in the preparation of commercial FBS abrogates most viral agents that may be present in raw FBS, but BVDV may pass through the filters because of its small size and its pleomorphism. While detection of bovine herpes virus-1 and parainfluenza-3 (PI-3) is determined by observation of the cytopathic effect, and also by hemadsorption in the case of PI-3, the most frequently isolated BVDV is non cytopathic, and infects cells without morphological alterations, inducing problems that arise after several cell generations. Batches of raw and processed FBS were analyzed. Frequencies of BVDV detection in raw serum in Argentina were similar to those published for USA. By conventional methods for BVDV detection, only 2 of 20 commercial batches of FBS had BVDV. Using cell cultures maintained with high concentrations of the serum under study for at least 2 weeks, with detection of viral antigen by indirect immunofluorescence, the percentage of BVDV detection was 80. This method shows that most lots of commercial FBS contain BVDV. RT-PCR allows faster detection of the viral genome, but it must be validated, as it does not show viral replication. To eliminate the problem of BVDV contamination in FBS, only gamma irradiated FBS is used in our laboratory.
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Índice:
LILACS (Américas)
Assunto principal:
Sangue
/
Vírus da Diarreia Viral Bovina
Limite:
Animais
Idioma:
Espanhol
Revista:
Rev. argent. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
CICVyA/AR
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