Comparación entre el método estándar sugerido por APHA y los métodos Simplate y Petrifilm, para la identificación del grupo coliforme y de Escherichia coli en tilapia (Oreochromis sp) procedente de piscicultura de agua dulce / Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water
Rev. argent. microbiol
;
32(1): 15-19, ene.-mar. 2000.
Artigo
em Espanhol
| LILACS
| ID: lil-332543
ABSTRACT
We have compared three methods for the detection and quantification of coliforms and E. coli in fish the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100 of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.
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Índice:
LILACS (Américas)
Assunto principal:
Contagem de Colônia Microbiana
/
Tilápia
/
Enterobacteriaceae
/
Infecções por Enterobacteriaceae
/
Escherichia coli
/
Doenças dos Peixes
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Espanhol
Revista:
Rev. argent. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2000
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Escola de Veterinária/BR
/
Fundação Ezequiel Dias/BR
/
Universidade Federal de Minas Gerais/BR
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