Estandarización y evaluación del ensayo vibriocida modificado / Standardization and evaluation of the modified vibriocidal assay
Rev. cuba. med. trop
;
51(3): 156-159, Sept.-Dec. 1999.
Artigo
em Espanhol
| LILACS
| ID: lil-333516
ABSTRACT
The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Vibrio cholerae
/
Atividade Bactericida do Sangue
Limite:
Animais
/
Humanos
Idioma:
Espanhol
Revista:
Rev. cuba. med. trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Cuba
Instituição/País de afiliação:
Instituto de Medicina Tropical Pedro Kouri/CU
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