Respuesta humoral específica en ratones Balb/c inoculados con una librería genómica de expresión de Trypanosoma cruzi / Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi
Rev. cuba. med. trop
;
51(1): 20-25, ene.-abr. 1999.
Artigo
em Espanhol
| LILACS
| ID: lil-333545
RESUMO
A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Anticorpos Antiprotozoários
/
Biblioteca Genômica
/
DNA de Protozoário
Limite:
Animais
Idioma:
Espanhol
Revista:
Rev. cuba. med. trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
1999
Tipo de documento:
Artigo
País de afiliação:
Cuba
Instituição/País de afiliação:
Instituto FINLAY/CU
/
Instituto de Medicina Tropical Pedro Kouri/CU
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