Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
Mem. Inst. Oswaldo Cruz
;
98(2): 269-271, Mar. 15, 2003. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-334266
ABSTRACT
We describe a method for culturing over 90 percent pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma
/
Técnicas de Cultura de Células
/
Macrófagos
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Universidad de Antioquia/CO
Similares
MEDLINE
...
LILACS
LIS