Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies
Mem. Inst. Oswaldo Cruz
;
98(2): 287-290, Mar. 15, 2003. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-334270
ABSTRACT
Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three æm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Psychodidae
/
Fixação de Tecidos
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2003
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Fiocruz/BR
/
Universidade Federal de Viçosa/BR
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