Human platelet nitric oxide synthase activity: an optimized method
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
38(3): 305-313, jul.-set. 2002. graf
Artigo
em Inglês
| LILACS
| ID: lil-334620
ABSTRACT
We investigated the kinetic analysis of human platelet Nitric Oxide Synthase (NOS) activity by the rate of conversion of [3H] arginine to [3H]-citrulline in unstimulated fresh platelets. NOS activity was present in the membrane fraction and cytosol, and was Ca²+ - and calmodulin dependent which is a characteristic of endothelial NOS. NOS activity was also dependent of NADPH since the omission of this cofactor induced an important decrease (85,2 por cento) in the enzyme activity. The kinetic varied with protein and arginine concentration but optimum concentrations were found up to 60 minutes, and up to 80 µg of protein at 120 nM of argine and 0.5 µCi of3H-arginine...
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Arginina
/
Plaquetas
/
Citrulina
/
Doenças Neurodegenerativas
/
Ativação Enzimática
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Inglês
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Assunto da revista:
Bioquímica
/
Farmácia
/
Farmacologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR
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