Identification of new isolates of Bacillus thuringiensis using rep-PCR products and d-endotoxin electron microscopy
Genet. mol. biol
;
25(2): 225-229, Jun. 2002. ilus
Artigo
em Inglês
| LILACS
| ID: lil-335793
ABSTRACT
PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Bacillus thuringiensis
/
Proteínas de Bactérias
/
Reação em Cadeia da Polimerase
/
Endotoxinas
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UNESP/BR
/
USP/BR
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