Unilateral enucleation induces an increase of 160 kd neurofilament in lateral geniculate nuclei synapses
Biocell
;
20(1): 55-59, Apr. 1996.
Artigo
em Inglês
| LILACS
| ID: lil-336005
RESUMO
Ultrastructural synaptic changes of retinal origin in the pars dorsalis lateral geniculate nuclei (dLGN) after enucleation have been studied in this laboratory, showing a filamentous hypertrophy with maximal expression at 4-6 days post-lesion in monkeys (Pecci Saavedra et al., 1970, 1971). The aim of this work was to elucidate the nature of the newly formed filament in dLGN in post-enucleated rats. Male Wistar rats were fixed with 4 paraformaldehyde plus 0.25 glutaraldehyde in 0.1M phosphate buffer, through the abdominal aorta after 3, 5, and 7 days postenucleation. Sections obtained were incubated with antibodies to the phosphorylated portion of the 160 Kd neurofilaments (1:3000) and anti-GFAP (1:25000). There was an increase in 160 Kd neurofilament staining in axons and degenerating nerve endings in dLGN, as well as a typical astroglial immunostained reaction. Our results show that the newly formed neurofilaments after deafferentation are of the 160 Kd type, commonly present in normal axons.
Buscar no Google
Índice:
LILACS (Américas)
Assunto principal:
Sinapses
/
Enucleação Ocular
/
Proteínas de Neurofilamentos
/
Corpos Geniculados
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
1996
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Universidad de Buenos Aires/AR
Similares
MEDLINE
...
LILACS
LIS